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The data describe all zooplankton species abundance values estimated at each rectangular midwater trawl (RMT 1+8) target and routine sampling site collected during the 2021 TEMPO voyage on R/V Investigator. During the TEMPO voyage, zooplankton were collected using the RMT 1+8 plankton net and sorted down to the lowest taxonomic level possible. The supporting data set, in CSV format, feature abundances estimated from RMT+1 samples only. The data file is named allzooplankton.csv. The fields (columns) in the file are: "Site" – sampling site ID for each routine (RXX) and target (TXX) trawl using RMT1+8. "Date" – date of each sample collected (ie. target or routine trawl) [dd/mm/yyyy]. "Latitude" – latitude of each trawl [degrees] (-dd.ddddd). "Longitude" – longitude of each trawl [degrees] (dd.ddddd). "Chla" – Integrated estimate of chlorophyll-a (mg m^-2) averaged at depths between 10 to 200 m, which aligns with the section of the water column where zooplankton were sampled. "Depth" – Bathymetric depth estimated at each sampling station. "MLD_03" – Mixed layer depth (m). Not used in final analysis. "MLD_05" – Mixed layer depth (m). Not used in final analysis. "MLD_N2max" – Mixed layer depth (m) where the N^2 maximum is found, i.e., the pycnocline. "CT_200" – Absolute temperature (°C) averaged at depths between surface and 200 m maximum net sampling depth. Values are derived from in situ CTD measurements at each sampling site. "SA_200" – Absolute salinity (g kg^-1) averaged at depths between surface and 200 m maximum net sampling depth. Values are derived from in situ CTD measurements at each sampling site. "IceMeltDays" – Number of days since sea ice melted calculated from daily passive microwave estimated percent sea ice concentration taken from the National Snow and Ice Data Centre. "m_to_Ice" – Distance (m) estimated from sampling site to edge of ice. "IceConc" – Actual ice concentration value (%). It is the percentage of an area covered in sea ice. The remaining columns are zooplankton species abundances (individuals per 1000 cubic metres) estimated from count data, volume of original sample, split of sample analysed under the microscope and calibration of the flowmeter: "R.gigas" – Rhincalanus gigas, copepod "C.propinquus" – Calanus propinquus, copepod "C.simillimus" – Calanus simillimus, copepod "C.acutus" – Calanoides acutus, copepod "Metridia sp." – Metridia species, copepod "M.gerlachei" – Metridia gerlachei, copepod "Paraeuchaeta sp." – Paraeuchaeta species, copepod "Pleuromamma sp." – Pleuromamma species, copepod "Oncaea" – Oncaea species, copepod "O.antarctica" – Oncaea antarctica, copepod "Neo tonsus" – Neocalanus tonsus, copepod "Neo gracilis" – Neocalanus gracilis, copepod "Stephos longipes" – Stephos longipes, copepod "Ctenocalanus sp." – Ctenocalanus species, copepod "Microcal pygmaeus" – Microcalanus pygmaeus, copepod "Oithona similis" – Oithona similis, copepod "Oithona frigida" – Oithona frigida, copepod "Heterhabdus australis" – Heterorhabdus australis, copepod "Heterhabdus sp." – Heterorhabdus species, copepod "Harpacticoid" – Harpacticoid species, copepod "Haloptilus sp." – Haloptilus species, copepod "Candacia sp." – Candacia species, copepod "Solecithricella sp." – Solecithricella species, copepod "Clausocalanus breviceps" – Clausocalanus breviceps, copepod "Clausocalanus laticeps" – Clausocalanus laticeps, copepod "Clausocalanus sp." – Clausocalanus species, copepod "Aetideops antarctica – Aetideopsis antarctica, copepod "Aetide minor" – Aetideopsis minro, copepod "Euchirella rostromanga" – Euchirella rostromanga, copepod "Heterostylites longicornis" – Heterostylites longicornis, copepod "Gaidius tenuispinus" – Gaidius tenuispinus, copepod "Gammaridean amphipod" – Gammaridean, amphipod "T.gaudichaudii" – Themisto gaudichaudii, amphipod "Parathemisto sp." – Parathemisto species, amphipod "Hyperia antarctica" – Hyperia antarctica, amphipod "Hyperia sp." – Hyperia species, amphipod "Hyperiella dilatata" – Hyperiella dilatata, amphipod "Hyperiella antarctica" – Hyperiella antarctica, amphipod "Hyperiella sp." – Hyperiella species, amphipod "Hyperiella macronyx" – Hyperiella macronyx, amphipod "Hyperiella dilatate" – Hyperiella dilatata, amphipod "Primno macropa" – Primno macropa, amphipod "Hyperoche medusarum" – Hyperoche medusarum, amphipod "E.crystallorophius" – Euphausia crystallorophius, krill "E.superba" – Euphausia superba, krill "T.macrura" – Thysanoessa macrura, krill "Krill (other)" – unidentified krill species "Decapod" – decapod species "L.h.antarctica" – Limacina helicina antarctica, pteropod "C.pyramidata" – Clio pyramidata, pteropod "Clione antarctica" – Clione antarctica, pteropod "S.australis" – Spongiobranchaea australis, pteropod "Gymnosome" – unidentified gymnosome species, pteropod "Ptero egg mass" – pteropod egg mass "Tiarrana rotunda" – Tiarrana rotunda, polychaete "Traviopsis/Typhloscoplex" – Traviopsis species or Typhloscoplex species, polychaete "Phalacrophorus pictus" – Phalacrophorus pictus, polychaete "Rhynchon brongraini" – Rhynchonerella brongraini, polychaete "Rhynchoneralla petersii" – Rhynchonerella petersii, polychaete "Rynchonerella sp." – Rhynchonerella species, polychaete "Vanadis sp." – Vanadis species, polychaete "Vanadis longissima" – Vanadis longissima, polychaete "Vanadis antarctica" – Vanadis antarctica, polychaete "Pelagobia longicerrata" – Pelagobia longicerrata, polychaete "Iopsilidae worm" – Iopsilidae species, polychaete "Polychaete" – unidentified polychaete worm "Furry worm" – unidentified "furry" polychaete worm "Larval worm" – unidentified "larval" polychaete worm "Tiny worm" – unidentified tiny-sized polychaete worm "Tomopteris septentrionalis" – Tomopteris septentrionalis, tomopteris "Tomopteris sp." – Tomopteris species, tomopteris "Phalacrophorus sp." – Phalacrophorus species, tomopteris "Isopod" – isopod worm "Tintinnids" – tintinnid species "Neogloboquadrina pachyderma" – Neogloboquadrina pachyderma, foraminifera "Siphonophore" – Siphonophore species "Appendicularian" – Appendicularian species "Ostracod" – unidentified ostracod species "Alacia hettacra" – Alacia hettacra, ostracod "Metaconchoecia skogsbergi" – Metaconchoecia skogsbergi, ostracod "Deeveyoecia arcuata" – Deeveyoecia arcuata, ostracod "Austrinoecia isocheira (<1mm)" – Austrinoecia isocheira ostracod, less than 1 mm in body size "Boroecoa antipoda" – Boroecoa antipoda, ostracod "Sagitta maxima" – Sagitta maxima, chaetognath "Sagitta serratodentata" – Sagitta serratodentata, chaetognath "Sagitta gazella" – Sagitta gazella, chaetognath "Sagitta marri" – Sagitta marri, chaetognath "Sagitta sp." – Sagitta species, chaetognath "Eukrohnia hamata" – Eukrohnia hamata, chaetognath "Unknwn chaetognath" – Unidentified species of chaeotognath "Fish egg" – fish egg "Larval fish" – unidentified species of larval fish "jelly nubby tentacles" – unidentified species of cnidarian (with "nubby" tentacles) "jelly w/no tentacles" – unidentified species of cnidarian (with no tentacles) "jelly" – unidentified species of cnidarian "TOTALS" – tally of total abundances across each row (total abundances across all zooplankton species for each sampling site)