https://data.aad.gov.au/ipt/resource?r=molecular_ecology Julie McInnes IMAS-Univeristy of Tasmania Investigator

Hobart TAS AU

julie.mcinnes@utas.edu.au Dave Connell Australia Antarctic Division

203 Channel Highway Hobart Tasmania 7050 AU

Dave.Connell@aad.gov.au Data Officer AADC Australia Antarctic Division

203 Channel Highway Hobart Tasmania 7050 AU

metadata@aad.gov.au https://data.aad.gov.au/ distributor 2018-10-02 eng This spreadsheet provides the sequences counts for the DNA groups found in the scats of black-browed albatross at New Island and Steeple Jason Island, Falkland Islands; Diego Ramírez and Albatross Islet, Chile; Bird Island, South Georgia; Canyon des Sourcils Noirs, Kerguelen Archipelago, France; Macquarie Island, Australia; and Campbell albatross at Campbell Island, NZ. Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages. Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology. Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. https://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2017_MolecularEcology OCEAN, SOUTHERN OCEAN, ATLANTIC OCEAN, INDIAN OCEAN, DIEGO RAMIREZ, CAMPBELL ISLAND, ALBATROSS ISLET, BIRD ISLAND, POLAR, -176.484 -66.094 -46.757 -59.889 2013-12-01 2016-01-15 Species of invertebrates were identified in fecals samples of Black-Browed Albatross by DNA metabarcoding species Thalassarche melanophris Black-Browed Albatross class Actinopterygii class Anthozoa class Cephalopoda class Chondrichthyes subclass Crustacea genus Ctenophora class Hydrozoa class Scyphozoa subphylum Tunicata unkown Julie McInnes IMAS-Univeristy of Tasmania Investigator

Hobart TAS AU

julie.mcinnes@utas.edu.au Rachel Anderman Department of Primary Industries, Parks, water and Environment Investigator

Hobart TAS AU

Ben Raymond Australia Antarctic Division Investigator

203 Channel Highway Hobart Tasmania 7050 AU

ben.raymond@aad.gov.au http://www.antarctica.gov.au/ Mary-anne Lea IMAS-University of Tasmania Investigator

Hobart TAS AU

MaryAnne.Lea@utas.edu.au http://www.utas.edu.au/profiles/staff/imas/mary-anne-lea Bruce Deagle Australia Antarctic Division Investigator

203 Channel Highway Hobart Tasmania 7050 AU

Bruce.Deagle@aad.gov.au http://www.antarctica.gov.au/ Scat samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA) Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages. Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology. See the referenced paper for more information. 2018-09-24 - dataset reformatted by Daniela Farias for GBIF/OBIS compliance. The original datasheet was reformatted to fit IPT Biodiversity.AQ standard. The new datasheet "McInnesMolecularEcology.csv" provides the dataset ID, event ID, recordNumber, resourceID, relatedResourceID, relationshipOfResource, relationshipAccordingTo, locationID, decimalLatitude, decimalLongitude, country, countryCode, eventDate, year, verbatimMonth, behaviour, occurrenceStatus, basisOfRecord, organismQuantity, organismQuantityType, protocol. The taxonomical organisation is provided to the lowest taxonomical rank that could be determined, after matched in WoRMS (World Register of Marine Species). Julie McInnes author This spreadsheet provides the sequences counts for the DNA groups found in the scats of black-browed albatross at New Island and Steeple Jason Island, Falkland Islands; Diego Ramírez and Albatross Islet, Chile; Bird Island, South Georgia; Canyon des Sourcils Noirs, Kerguelen Archipelago, France; Macquarie Island, Australia; and Campbell albatross at Campbell Island, NZ. Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages. Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology. Australian Antarctic Science Grant, Grant/ Award Number: 4014 and 4112; Winifred Violet Scott Charitable Trust; Falkland Islands Government; FCT—Portugal, Grant/ Award Number: UID/MAR/04292/2013 2018-09-18T10:51:34.446+00:00 dataset McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. 2017 High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology.