Abatus Microsatellites data set 2009/13

Occurrence
Latest version published by Australian Antarctic Data Centre on Mar 5, 2020 Australian Antarctic Data Centre

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Description

The present data set corresponds to the genotypes for seven microsatellite markers for three Antarctic sea urchin species of the genus Abatus. Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania.

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 249 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Carrea, C., Burridge, C., King, C.K., Miller, K. (2016, updated 2018) Abatus Microsatellites data set Australian Antarctic Data Centre -

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Australian Antarctic Data Centre. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 852fd378-2356-4b27-be1b-e7c05aa3ed36.  Australian Antarctic Data Centre publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Atlas of Living Australia.

Keywords

Occurrence; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; ECHINODERMS; SEA URCHINS

Contacts

Karen Miller
  • Originator
  • Point Of Contact
  • Principal Investigator
Australian Institute of Marine Science
AU
Cecilia Carrea
  • Originator
  • Point Of Contact
  • Author
University of Tasmania
7005 Hobart
Tasmania
AU
Christopher Burridge
  • Originator
  • Investigator
University of Tasmania
7005 Hobart
Tasmania
AU
Catherine King
  • Originator
  • Investigator
Australian Institute of Marine Science
Perth
Western Australia
AU
Dave Connell
  • Metadata Provider
Australia Antarctic Division
  • 203 Channel Highway
7050 Hobart
Tasmania
AU
Data Officer
  • Distributor
  • Scientific Data Coordinator
Australia Antarctic Division
  • 203 Channel Highway
7050 Hobart
Tasmania
AU

Geographic Coverage

Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica.

Bounding Coordinates South West [-68.578, -77.959], North East [-68.566, 58.359]

Taxonomic Coverage

Main goal was to study and compare the fine-scale population structure and demographic history of three species of Antarctic sea urchins (Abatus ingens, Abatus shackletoni and Abatus philippii) in the near-shore area surrounding the Australian Reseach Sation Davis in East Antarctica. A secondary goal was to test potential impacts of pollution from the Davis Station wastewater discharge on the genetic diversity and effective population size of these species

Species Abatus ingens, Abatus shackletoni, Abatus philippii

Temporal Coverage

Start Date / End Date 2009-10-01 / 2013-03-31

Project Data

The present data set corresponds to the genotypes for seven microsatellite markers for three Antarctic sea urchin species of the genus Abatus. Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania.

Title Abatus Microsatellites data set
Identifier AAS_3051_AbatusMicrosatellites
Funding This project was supported by the Australian Government through its Australian Antarctic Science Grants Program, awarded to K.J.M. (Project 3051) and C.K.K. (Project 4100)
Study Area Description Near-shore area surrounding the Australian Research Station Davis in East Antarctica
Design Description Original datasheet was reformatted to fit GBIF/ IPT Biodiversity.AQ standard. The new datasheet "AbatusUrchin_microsatellites.csv" provides the dataset ID, verbatim latitude and verbatim longitude that were converted to decimal latitude and longitude, also provided. Taxonomical organisation to the lowest taxonomical rank that could be determined, after matched in WoRMS (World Register of Marine Species). Also provides other parameters such as localisation, sampling depth, occurrence, and date of event.

The personnel involved in the project:

Karen Miller

Sampling Methods

Samples were collected from 5 sites, and the largest distance between any 2 sampling sites was 5 km. Three of the sites are considered heavily impacted: OF (outfall), OF1 and OF2 are within 500 m of the outfall pipe. The 2 control sites are considered to have negligible impact from the wastewater discharge; OF3 is located ~2.4 km from the outfall pipe to the northeast, and OF4 ~2.6 km to the southwest. Moreover, the OF3 and OF4 sites are both removed from the predominant longshore flow that transports the wastewater plume southeastwards along the coast (Stark et al. unpubli.). Samples from OF1 and OF2 were collected at 4.5− 7.0 m and 10−12 m depths, respectively, during the 2009/2010 Antarctic field season, and samples from OF, OF3 and OF4 were taken at depths <4 m during the 2012/2013 season.

Study Extent Individuals of 3 species of Abatus were collected in the near-shore area surrounding Davis Station (68°34’36’’S, 77°58’03’’E) located on Prydz Bay, Vestfold Hills, in Princess Elizabeth Land, East Antarctica.
Quality Control Samples from sampling sites OF1 and OF2 were collected during the 2009/2010 summer season. Samples from sampling sites OF, OF3 and OF4 were collected during the 2012/2013 summer season. Depths at which samples were collected: OF1: 4.5-7.0 m deep OF2: 10-12 m deep OF, OF3, OF4 equal or less than 4 m deep.

Method step description:

  1. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania.

Bibliographic Citations

  1. Cecilia Carrea, Christopher P. Burridge, Catherine K. King, Karen J. Miller (2016) Population structure and long-term decline in three species of heart urchins Abatus spp. near-shore in the Vestfold Hills region, East Antarctica. Marine Ecology Progress Series, 545:227-238 doi: 10.3354/meps11573

Additional Metadata

Marine, harvested by OBIS

Alternative Identifiers 852fd378-2356-4b27-be1b-e7c05aa3ed36
https://data.aad.gov.au/ipt/resource?r=abatusmicrosatellite2009_13