Abatus Microsatellites data set 2009/13

資料紀錄

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如何引用

研究者應依照以下指示引用此資源。:

Carrea, C., Burridge, C., King, C.K., Miller, K. (2016, updated 2018) Abatus Microsatellites data set Australian Antarctic Data Centre -

權利

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此資料的發布者及權利單位為 Australian Antarctic Data Centre。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 852fd378-2356-4b27-be1b-e7c05aa3ed36。  Australian Antarctic Data Centre 發佈此資源，並經由Atlas of Living Australia同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; ECHINODERMS; SEA URCHINS

聯絡資訊

地理涵蓋範圍

Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica.

分類群涵蓋範圍

Main goal was to study and compare the fine-scale population structure and demographic history of three species of Antarctic sea urchins (Abatus ingens, Abatus shackletoni and Abatus philippii) in the near-shore area surrounding the Australian Reseach Sation Davis in East Antarctica. A secondary goal was to test potential impacts of pollution from the Davis Station wastewater discharge on the genetic diversity and effective population size of these species

時間涵蓋範圍

計畫資料

The present data set corresponds to the genotypes for seven microsatellite markers for three Antarctic sea urchin species of the genus Abatus. Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania.

參與計畫的人員:

取樣方法

Samples were collected from 5 sites, and the largest distance between any 2 sampling sites was 5 km. Three of the sites are considered heavily impacted: OF (outfall), OF1 and OF2 are within 500 m of the outfall pipe. The 2 control sites are considered to have negligible impact from the wastewater discharge; OF3 is located ~2.4 km from the outfall pipe to the northeast, and OF4 ~2.6 km to the southwest. Moreover, the OF3 and OF4 sites are both removed from the predominant longshore flow that transports the wastewater plume southeastwards along the coast (Stark et al. unpubli.). Samples from OF1 and OF2 were collected at 4.5− 7.0 m and 10−12 m depths, respectively, during the 2009/2010 Antarctic field season, and samples from OF, OF3 and OF4 were taken at depths <4 m during the 2012/2013 season.

方法步驟描述:

1. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania.

引用文獻

1. Cecilia Carrea, Christopher P. Burridge, Catherine K. King, Karen J. Miller (2016) Population structure and long-term decline in three species of heart urchins Abatus spp. near-shore in the Vestfold Hills region, East Antarctica. Marine Ecology Progress Series, 545:227-238 doi: 10.3354/meps11573

額外的詮釋資料