Venom on ice: Novel venoms from Antarctic marine animals 2007/08

Registro biológico
Última versión publicado por Australian Antarctic Data Centre el oct 2, 2018 Australian Antarctic Data Centre
Fecha de publicación:
2 de octubre de 2018
Licencia:
CC-BY 4.0

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Descripción

Metadata record for data from ASAC Project 2897 See the link below for public details on this project. Public The aim of this multi-disciplinary proposal is to examine the molecular evolution of toxic proteins across the full taxonomical spectrum of venomous Antarctic marine animals. The project will create a comparative encyclopedia of the evolution of the venom system in the Antarctic marine animal kingdom and elucidate the underlying structure-function relationships between these toxic proteins. Through a process utilising cutting edge analytical techniques, such as cDNA cloning and molecular modelling, a feedback loop of bioactivity testing will be created to contribute substantially towards the area of drug design and development from toxic animal peptides. Project objectives: The aim of this project is to investigate the evolution of the molecular, structural and functional properties of Antarctic marine animal venom systems. This integrative project aims to investigate the origin and evolution of secreted proteins in the venom glands of toxic polar animals by means of: - Analysis of mechanisms of evolution in multigene families. - Phylogenetic analysis of evolutionary relationships among secreted proteins in the venom glands of major lineages; - Search for correlations between: (i) evolution of venom gland structure (ii) molecular evolution of venom components, and (iii) ecological specialisation of the animal - Bioactivity studies will be conducted upon representative purified or synthesised proteins. - A first ever comparison of the convergent strategies between Arctic and Antarctic endemic fauna. The results will help us to understand protein evolution, will cast light on the classic problem of how venom systems evolve, and may provide leads in the search for commercially-exploitable venom proteins. Taken from the 2008-2009 Progress Report: Progress against objectives: We have completed the genetic analyses of the specimens and sequence analyses. Phylogenetic positioning is robust other than a few deep level nodes. We are undertaking a second round of genetic analyses using different primers in order to resolve these nodes. Biochemical analyses of crude protein secretions from the posterior salivary (venom) glands has revealed temperature specific modifications of some of the venom components to adapt them to the polar conditions. We have tested the secretions in a battery of assays. We are now repeating those assays using purified proteins in order to determine which types are responsible for particular effects and also investigate synergistic interactions. Taken from the 2009-2010 Progress Report: Progress against objectives: We have undertaken genetic analyses of the specimens collected, and investigated specific adaptations of their venom systems. Results to-date include: - Antarctic octopuses are more genetically diverse than previously appreciated, including at least one new genus - an inverse relationship exists between the size of the venom gland and the size of the beak - their venoms have undergone temperature-specific adaptationsQuality: The values provided in temporal and spatial coverage are approximate only. Taken from the 2008-2009 Progress Report: Field work: We conducted fieldwork to obtain octopus specimens from the northern limit in Norwegian sub-polar waters. Laboratory activity/analysis: We have used the COI genetic marker to examine the phylogenetic relationships of the specimens collected. This marker was adequate for some nodes but not others. Thus other genetic markers are being evaluated for relative usefulness. We have also conducted different assays to examine the enzymatic properties of the venoms. In the next year we will use reverse phase HPLC to purify the crude venoms into individual toxins and examine their properties. We will also commence cDNA library construction in order to obtain full-length sequences of the toxins. This will allow for comparison with those we have obtained from temperate and tropical species with the aim of identifying regions that confer temperature specific activity. Taken from the 2009-2010 Progress Report: Laboratory activity/analysis - genetic fingerprinting of each specimen collecting - analysis of temperature-specific adaptations of different enzyme types present in the venoms

Registros

Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 14 registros.

Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Fry, B.G., Hodgson, W. and Norman, J. (2007, updated 2017) Venom on ice: Novel venoms from Antarctic marine animals. - CAASM Metadata

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es Australian Antarctic Data Centre. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 59997fb4-d940-4305-90b4-6dee190260bd.  Australian Antarctic Data Centre publica este recurso y está registrado en GBIF como un publicador de datos avalado por Atlas of Living Australia.

Palabras clave

Occurrence

Datos externos

Los datos del recurso también están disponibles en otros formatos

Point of truth metadata record https://data.aad.gov.au/metadata/records/ASAC_2897 ASCII DIF 9.9

Contactos

BRYAN FRY
  • Punto De Contacto
  • INVESTIGATOR
  • University of Queensland
4072 Brisbane
QLD
AU
DAVE CONNELL
  • Proveedor De Los Metadatos
  • DIF AUTHOR
  • Australian Antarctic Division 203 Channel Highway
7050 Kingston
Tasmania
AU
BRYAN FRY
  • Punto De Contacto
  • TECHNICAL CONTACT
  • University of Queensland
4072 Brisbane
QLD
AU
WAYNE HODGSON
  • Punto De Contacto
  • TECHNICAL CONTACT
  • Monash University
3800
Victoria
AU
JANETTE NORMAN
  • Punto De Contacto
  • TECHNICAL CONTACT
  • Southern Cross University National Marine Science Centre
4321 Coffs Harbour
NSW
AU
DATA OFFICER AADC
  • Distribuidor
  • DATA CENTER CONTACT
Australian Antarctic Data Centre, Australia
  • Australian Antarctic Division 203 Channel Highway
7050 Kingston
Tasmania
AU

Cobertura geográfica

OCEAN > SOUTHERN OCEAN CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR

Coordenadas límite Latitud Mínima Longitud Mínima [-70, -180], Latitud Máxima Longitud Máxima [-60, 180]

Cobertura taxonómica

Species of venomous Antarctic Marine animals identified

Género Benthoctopus, Cirroctopus, Pareledone
Especie Adelieledone polymorpha, Megaleledone setebos, Pareledone aequipapillae, Pareledone aurata, Pareledone cornuta, Pareledone panchroma, Pareledone subtilis, Pareledone turqueti, Thaumeledone peninsulae

Cobertura temporal

Fecha Inicial / Fecha Final 2007-09-30 / 2010-03-31

Referencias bibliográficas

  1. Undheim EA, Georgieva DN, Thoen HH, Norman JA, Mork J, Betzel C, Fry BG. (2010) Venom on ice: first insights into Antarctic octopus venoms.. . 56(6), 897-913 doi:10.1016/j.toxicon.2010.06.013
  2. Undheim EA, Norman JA, Thoen HH, Fry BG. (2010) Genetic identification of Southern Ocean octopod samples using mtCOI.. . 333(5), 395-404 doi:10.1016/j.crvi.2010.02.002

Metadatos adicionales