Metadata details
AAS_4026_Variance_Experiment
All scientific data collected by the Australian Antarctic program (AAp) are eventually described in the Catalogue of Australian Antarctic and Subantarctic Metadata (CAASM). CAASM can be used to search through AAp data descriptions, and it also provides links to access publicly available datasets, which can either be immediately downloaded or obtained from the Australian Antarctic Data Centre (AADC).
- Citation
- Deppeler, S., Hancock, A. and Davidson, A. (2020) Data for flow cytometry, FlowCam, and microscopy comparison experiment, Ver. 1, Australian Antarctic Data Centre - doi:10.4225/15/5b235b91a7143, Accessed: 2026-05-22
- Title
- Data for flow cytometry, FlowCam, and microscopy comparison experiment
- Data Centre
- Australian Antarctic Data Centre, Australia
- DOI
- doi:10.4225/15/5b235b91a7143
- Created Date
- 2018-06-11
- Revision Date
- 2025-06-26
- Parent record
- AAS_4026_Ocean_Acidification_Marine_Microbes_Parent
Datasets and documents
Related links
Description
This data set was collected from two minicosm experiments conducted at Davis Station, Antarctica.
1. Variance experiment - 2013/14 summer season
2. Ocean acidification experiment - 2014/15 summer season
It includes:
- description of methods for all data collection and analyses.
- environmental data logged throughout the experiment; nutrients, temperature, light climate.
- flow cytometry counts; autotrophic cells, heterotrophic nanoflagellates, and prokaryotes.
- FlowCam counts; individual phytoplankton species data.
- microscopy counts; individual phytoplankton species data.
Context from the public summary for project 4026:
Manmade CO2 has increased ocean acidity by 30% and it is projected to rise 300% by 2100. Antarctic waters will be amongst the earliest and most severely affected by this increase. Microbes are the base of the marine food chain and primary drivers of the biological pump. This project will incubate natural communities of Antarctic marine microbes in minicosms at a range of CO2 concentrations to quantify changes in their structure and function, the physiological responses that drive these changes, and provide input to models that predict effects on biogeochemical cycles and Antarctic food webs.
Purpose
The purpose of this data set is to compare the results of three commonly used techniques for microbial community composition and abundance counts.
Quality
The minicosm experimental design measured the microbial community growth in six unreplicated fCO2 treatments. Therefore, sub-samples from each minicosm were within-treatment pseudoreplicates and thus, results of statistical analysis must be interpreted conservatively.
Access
These data are publicly available for download from the provided URL.
Temporal Coverages
- Start date: 2013-11-01 - Stop date: 2015-02-28
Spatial Coverages
Science Keywords
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
- EARTH SCIENCE > CLIMATE INDICATORS > ATMOSPHERIC/OCEAN INDICATORS > OCEAN ACIDIFICATION
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > FLAGELLATES > DINOFLAGELLATES
Additional Keywords
- OCEAN ACIDIFICATION
- FLOWCAM
Locations
- GEOGRAPHIC REGION > POLAR
- OCEAN > SOUTHERN OCEAN > PRYDZ BAY
- CONTINENT > ANTARCTICA > DAVIS STATION
Platforms
- FIELD INVESTIGATION
- LABORATORY
Instruments
- FLOW CYTOMETRY
- MICROSCOPES
Researchers
- deppeler, stacy (INVESTIGATOR,TECHNICAL CONTACT,DIF AUTHOR)
- hancock, alyce (INVESTIGATOR)
- davidson, andrew (INVESTIGATOR)
Use Constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026_Variance_Experiment when using these data.
