Metadata details

AAS_4406_BP_MP_2005

All scientific data collected by the Australian Antarctic program (AAp) are eventually described in the Catalogue of Australian Antarctic and Subantarctic Metadata (CAASM). CAASM can be used to search through AAp data descriptions, and it also provides links to access publicly available datasets, which can either be immediately downloaded or obtained from the Australian Antarctic Data Centre (AADC).

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Citation
Ferrari, B.C. (2024) Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005), Ver. 1, Australian Antarctic Data Centre - doi:10.26179/5e33a1e1e8c87, Accessed: 2026-09-16
Title
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)
Data Centre
Australian Antarctic Data Centre, Australia
DOI
doi:10.26179/5e33a1e1e8c87
Created Date
2020-01-31
Revision Date
2024-09-13
Parent record
None

Datasets and documents

    Related links

    Description

    Sample collection:
    All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below.

    Metagenome sequencing and assembly:
    Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample.

    Description of ACE Sample IDs (including in filenames) and the relevant soil samples:
    * SB9748
    - Soil barcode= 91062
    - Original barcode= 91604
    - Sample Site= Browning Peninsula
    - Transect= 3
    - Distance samples= 2m
    - Concentration of DNA sent= 1.2ng/µL (required concentration)
    - Volume DNA sent= 80µL
    * SB9749
    - Soil barcode= 91604
    - Original barcode= 26587
    - Sample Site= Browning Peninsula
    - Transect= 3
    - Distance samples= 102m
    - Concentration of DNA sent= 1ng/µL (required concentration)
    - Volume DNA sent= 80µL
    * SB9750
    - Soil barcode= 91606
    - Original barcode= 26617
    - Sample Site= Browning Peninsula
    - Transect= 3
    - Distance samples= 202m
    - Concentration of DNA sent= 0.3ng/µL (required concentration)
    - Volume DNA sent= 180µL
    * SB9751
    - Soil barcode= 91447
    - Original barcode= 36260
    - Sample Site= Mitchell Peninsula
    - Transect= 2
    - Distance samples= 2m
    - Concentration of DNA sent= 0.86ng/µL (required concentration)
    - Volume DNA sent= 80µL
    * SB9752
    - Soil barcode= 91477
    - Original barcode= 36290
    - Sample Site= Mitchell Peninsula
    - Transect= 2
    - Distance samples= 102m
    - Concentration of DNA sent= 0.9ng/µL (required concentration)
    - Volume DNA sent= 80µL
    * SB9753
    - Soil barcode= 91507
    - Original barcode= 36320
    - Sample Site= Mitchell Peninsula
    - Transect= 2
    - Distance samples= 202m
    - Concentration of DNA sent= 1.9ng/µL (required concentration)
    - Volume DNA sent= 80µL

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    Quality

    No problems have been encountered with this dataset, but analysis is still ongoing.

    Access

    These data are publicly available for download from the provided URL.

    Temporal Coverages

    Spatial Coverages

    Latitude

    Northernmost:
    -66.20709
    Southernmost:
    -66.38156

    Longitude

    Westernmost:
    110.42908
    Easternmost:
    110.68176

    Science Keywords

    Additional Keywords

    • METAGENOMIC
    • SOIL
    • SOIL MICROBIOME
    • MICROBIAL COMMUNITY ANALYSIS

    Locations

    • GEOGRAPHIC REGION > POLAR
    • CONTINENT > ANTARCTICA > MITCHELL PENINSULA
    • CONTINENT > ANTARCTICA > BROWNING PENINSULA
    • CONTINENT > ANTARCTICA > WINDMILL ISLANDS
    • CONTINENT > ANTARCTICA > EAST ANTARCTICA

    Platforms

    • FIELD SURVEYS
    • LABORATORY

    Instruments

    • Automated DNA Sequencer

    Researchers

    • ferrari, belinda (INVESTIGATOR,TECHNICAL CONTACT)
    • ray, angelique (TECHNICAL CONTACT,DIF AUTHOR)

    Use Constraints

    This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

    Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4406_BP_MP_2005 when using these data.

    Creative Commons License