All scientific data collected by the Australian Antarctic program (AAp) are eventually described in the Catalogue of Australian Antarctic and Subantarctic Metadata (CAASM). CAASM can be used to search through AAp data descriptions, and it also provides links to access publicly available datasets, which can either be immediately downloaded or obtained from the Australian Antarctic Data Centre (AADC).
Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.
We tested the efficacy of DNA analysis to identify burrowing petrel species in a rapidly changing landscape, on a remote sub-Antarctic island following pest eradication. Using these data, we identify species presence and mapped the locations where species were detected with DNA metabarcoding.
See the methodology document in the download file for more information.
These data are publicly available for download from the provided URL.
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.
2021-04-210 - record created by Julie McInnes.