Metadata details
BROKE-West_flow_cytometry
All scientific data collected by the Australian Antarctic program (AAp) are eventually described in the Catalogue of Australian Antarctic and Subantarctic Metadata (CAASM). CAASM can be used to search through AAp data descriptions, and it also provides links to access publicly available datasets, which can either be immediately downloaded or obtained from the Australian Antarctic Data Centre (AADC).
- Citation
- Thomson, P.G. (2010) BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006, Ver. 1, Australian Antarctic Data Centre - doi:10.4225/15/59890669dda1a, Accessed: 2026-05-15
- Title
- BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006
- Data Centre
- Australian Antarctic Data Centre, Australia
- DOI
- doi:10.4225/15/59890669dda1a
- Created Date
- 2006-05-19
- Revision Date
- 2017-08-08
- Expected Date of Data Release
- 2010-08-20
- Data Version
- 1
- Parent record
- BROKE-West
Datasets and documents
Related links
Description
This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11.
Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA.
The fields in this dataset are:
Latitude
Longitude
Transect Number
CTD number, flow file
Depth (m)
Total bacteria (per millilitre)
Active bacteria (per millilitre)
Dead bacteria (per millilitre)
Protozoa (per millilitre)
Phytoplankton (per millilitre)
This work was completed as part of ASAC project 40 (ASAC_40).
Access
These data are publicly available for download from the provided URL.
Temporal Coverages
- Start date: 2006-01-10 - Stop date: 2006-02-27
Spatial Coverages
Science Keywords
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON
- EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC
Additional Keywords
- BROKE-West
- Flow cytometry
- bacteria
- FACScan Flow Cytometer
- Latitude
- Longitude
- Transect Number
- CTD
- Depth
- Total bacteria
- Active bacteria
- Dead bacteria
- Protozoa
- Phytoplankton
Locations
- OCEAN > SOUTHERN OCEAN
- CONTINENT > ANTARCTICA
- GEOGRAPHIC REGION > POLAR
Platforms
- SHIPS
- R/V Aurora Australis
Instruments
- FLOW CYTOMETRY
Researchers
- thomson, paul (INVESTIGATOR,TECHNICAL CONTACT,DIF AUTHOR)
Use Constraints
This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry when using these data.
Project
ISO Topic
- BIOTA
- OCEANS
Dataset Language
- English
Orignating Centre
- Australian Antarctic Division
Dataset Progress
- COMPLETE
IDN Node
- AMD/AU
- CEOS
- AMD
- ACE/CRC
Metadata Revision History
2010-08-20 - record updated by Dave Connell to publicly release the data. 2017-08-08 - record updated by Dave Connell - basic updates.
