Metadata details
soil_bacteria_fungi
All scientific data collected by the Australian Antarctic program (AAp) are eventually described in the Catalogue of Australian Antarctic and Subantarctic Metadata (CAASM). CAASM can be used to search through AAp data descriptions, and it also provides links to access publicly available datasets, which can either be immediately downloaded or obtained from the Australian Antarctic Data Centre (AADC).
- Citation
- Siciliano, S.D., Palmer, A.S., Winsley, T., Lamb, E., Bissett, A., Brown, M.V., van Dorst, J., Ji, M., Ferrari, B.C., Grogan, P., Chu, H. and Snape, I. (2014) Polar soil bacterial and fungal biodiversity survey, Ver. 1, Australian Antarctic Data Centre - doi:10.4225/15/526F42ADA05B1, Accessed: 2026-05-14
- Title
- Polar soil bacterial and fungal biodiversity survey
- Data Centre
- Australian Antarctic Data Centre, Australia
- DOI
- doi:10.4225/15/526F42ADA05B1
- Created Date
- 2013-10-27
- Revision Date
- 2019-07-25
- Parent record
- None
Datasets and documents
Related links
Description
It contains the raw sequences (.fasta files) quality scores (.qual files), flowgram data from the 454 instrument (.flow files), an index of each sequence read and the sample to which it belongs (.groups file) which can be read into the software package 'mothur' (www.mothur.org). This is provided for both the bacterial fraction (16S) and the fungal fraction (ITS).
These files were generated using the Roche 454 Titanium platform on 223 soils from the 8 locations previously mentioned. The data are generated using the 16S primers 27F and 519R and the fungal data are from the primers ITS1F and ITS 4.
In the referenced study the data were analysed in mother version 1.23.1.
In addition, the chemical and geographical data is provided as an excel spreadsheet. These parameters were all recorded but not necessarily implemented in the structural equation modelling. They are also the raw data and were transformed to reduce skewness and kurtosis.
Full method details are in the manuscript.
Quality
Statement of Authorship: SDS and IS designed, implemented and oversaw the multiyear study with SDS analyzing the data and writing the manuscript. AB & MVB analysed the sequencing data and contributed to writing the manuscript, JVD and MJ performed DNA extraction and ARISA analysis. AP collected the soil chemistry data and insured quality control of the entire data set. BF performed the sequencing on the Antarctic data set. PG coordinated the circumpolar arctic heath dataset and contributed to writing the manuscript, HC performed the biogeochemistry on that dataset. TW analysed the sequencing data and assisted in the meta-model analysis. EL helped develop and evaluate the SEM and contributed to writing the manuscript.
Access
These data are publicly available from the provided URL.
Temporal Coverages
- Start date: 2005-01-01 - Stop date: 2012-12-31
Spatial Coverages
Science Keywords
- EARTH SCIENCE > LAND SURFACE > SOILS > MICROFAUNA
- EARTH SCIENCE > LAND SURFACE > SOILS > MICROFLORA
- EARTH SCIENCE > LAND SURFACE > SOILS > SOIL CHEMISTRY
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
- EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > FUNGI
Additional Keywords
- Soil Bacteria
- Soil Fungi
Locations
- GEOGRAPHIC REGION > POLAR
- CONTINENT > ANTARCTICA
- CONTINENT > NORTH AMERICA
- CONTINENT > EUROPE
Platforms
- FIELD INVESTIGATION
- FIELD SURVEYS
- LABORATORY
Instruments
- Automated DNA Sequencer
Researchers
- siciliano, steven (INVESTIGATOR,TECHNICAL CONTACT)
- palmer, anne (INVESTIGATOR,TECHNICAL CONTACT)
- winsley, tristrom (INVESTIGATOR,TECHNICAL CONTACT,DIF AUTHOR)
- lamb, eric (INVESTIGATOR,TECHNICAL CONTACT)
- bissett, andrew (INVESTIGATOR,TECHNICAL CONTACT)
- brown, mark (INVESTIGATOR,TECHNICAL CONTACT)
- van dorst, josie (INVESTIGATOR,TECHNICAL CONTACT)
- ji, mukan (INVESTIGATOR,TECHNICAL CONTACT)
- ferrari, belinda (INVESTIGATOR,TECHNICAL CONTACT)
- grogan, paul (INVESTIGATOR,TECHNICAL CONTACT)
- chu, haiyan (INVESTIGATOR,TECHNICAL CONTACT)
- snape, ian (INVESTIGATOR,TECHNICAL CONTACT)
Use Constraints
This data set conforms to the CCBY Attribution License
(http://creativecommons.org/licenses/by/4.0/).
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=soil_bacteria_fungi when using these data.
