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Citation
Mcinnes, J.C. and Shaw, J. (2021) Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018., Ver. 1, Australian Antarctic Data Centre - doi:10.26179/5bkj-yg59, Accessed: 2021-12-02
Title
Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018.
Data Centre
Australian Antarctic Data Centre, Australia
DOI
doi:10.26179/5bkj-yg59
Created Date
2021-04-20
Revision Date
2021-04-20
Parent record
None

Description

Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.

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Purpose

We tested the efficacy of DNA analysis to identify burrowing petrel species in a rapidly changing landscape, on a remote sub-Antarctic island following pest eradication. Using these data, we identify species presence and mapped the locations where species were detected with DNA metabarcoding.

Quality

See the methodology document in the download file for more information.

Access

These data are publicly available for download from the provided URL.

Temporal Coverages

Spatial Coverages

Science Keywords

Additional Keywords

  • MISEQ
  • LC480
  • AAD GENTICS LAB
  • DNA METABARCODING
  • PROCELLARIIDAE
  • PELECANOIDES
  • RECOLONISATION
  • BURROWING PETRELS

Locations

  • OCEAN > SOUTHERN OCEAN
  • GEOGRAPHIC REGION > POLAR

Platforms

  • FIELD SURVEYS
  • LABORATORY

Instruments

  • Automated DNA Sequencer

Researchers

  • mcinnes, julie (INVESTIGATOR,TECHNICAL CONTACT,DIF AUTHOR)
  • shaw, justine (INVESTIGATOR,TECHNICAL CONTACT)

Use Constraints

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.

Creative Commons License